by williamsu | Aug 22, 2012 | Product News
Whatman Optitran Nitrocellulose Membranes
Reinforced nitrocellulose membranes
Optitran™ membrane consists of 100% pure nitrocellulose supported by an inert polyester nonwoven material within the membrane. The support does not affect transfer conditions or results and gives the membrane exceptional handling characteristics, allowing it to be reprobed repeatedly.
The Optitran nitrocellulose membrane provides high sensitivity with very low nonspecific binding. Using standard nitrocellulose protocols, stringent washing and blocking conditions are not necessary.
Optitran membranes combine flexibility, strength, and excellent signal-to-noise ratios, which are especially beneficial when experiments involve repeated stripping and reprobing.
Optitran supported nitrocellulose membranes combine sensitivity, strength, and savings.
Optitran binding capacity: 75 to 90 µg/cm2; autoclavable (liquid cool cycle)
| Ordering Information – Optitran Nitrocellulose Membranes |
| Catalog Number |
Description |
|
|
| 10439391 |
BA-S 83 Optitran, 0.2 µm, 20 x 20 cm, sheets, 25/pk |
| 10439380 |
BA-S 83 Optitran, 0.2 µm, 30 x 60 cm, sheets, 5/pk |
| 10439394 |
BA-S 83 Optitran, 0.2 µm, 20 cm x 3 m, sheets, 1/pk |
| 10439396 |
BA-S 83 Optitran, 0.2 µm, 30 cm x 3 m, roll, 1/pk |
|
|
| 10439116 |
BA-S 85 Optitran, 0.45 µm, 82 mm, circles, 50/pk |
| 10439126 |
BA-S 85 Optitran, 0.45 µm, 132 mm, circles, 50/pk |
| 10439191 |
BA-S 85 Optitran, 0.45 µm, 20 x 20 cm, sheets, 25/pk |
| 10439180 |
BA-S 85 Optitran, 0.45 µm, 30 x 60 cm, sheets, 5/pk |
| 10439194 |
BA-S 85 Optitran, 0.45 µm, 20 cm x 3 m, roll, 1/pk |
| 10439196 |
BA-S 85 Optitran, 0.45 µm, 30 cm x 3 m, roll, 1/pk |
by williamsu | Aug 22, 2012 | Product News
100% Pure Nitrocellulose Membranes
Protran™ nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible.
Other membranes referred to as ‘nitrocellulose’ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric, and fluorescent.
Whatman Protran Nitrocellulose Membranes Unlike PVDF membranes, Protran nitrocellulose does not require a methanol prewetting step. This makes Protran the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
Whatman Protran Nitrocellulose Membranes High binding, low background
In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.
High retention of small proteins
The 0.2 µm pore size Protran (BA83) nitrocellulose membrane has high surface area, ensuring binding of small proteins below 20 kD by reducing ‘blow-through’. The 0.45 µm pore size membrane (BA85) is suitable for larger molecular weight samples. BA79, with 0.1 µm pore size, is the membrane of choice for smaller proteins below 7 kD.
A key benefit of the proprietary Protran nitrocellulose formula is the proven shelf life of bound proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran.
Protran blotting sandwiches
A precut nitrocellulose membrane and 2 sheets of 3MM Chr blotting paper are prepackaged into a sandwich to help save time. This is made with our Protran BA83 or BA85, the highest quality NC membranes available for blotting.
For orders for NC membranes from Canada, please add a C to the end of the catalog number, for example 10485376C
Protran nitrocellulose membrane binding capacity: 80 to 150 µg/cm2; autoclavable (liquid cool cycle)
| Ordering Information – Protran Nitrocellulose Membranes |
| Catalog Number |
Description |
| Protran BA83 Blotting Sandwiches |
|
| 10485376 |
BA83 Protran, 0.2 µm, blotting sandwich, 7 x 8.5 cm, 20/pk |
| Protran BA85 Blotting Sandwiches |
|
| 10485374 |
BA85 Protran, 0.45 µm, blotting sandwich, 7 x 8.5 cm, 20/pk |
|
|
| 10402088 |
BA79 Protran, 0.1 µm, blotting membrane, 10.2 x 13.3 cm, sheet, fits Minifold 1 System, 10/pk |
| 10402062 |
BA79 Protran, 0.1 µm, blotting membrane, 20 x 20 cm, sheet, 5/pk |
| 10402091 |
BA79 Protran, 0.1 µm, blotting membrane, 20 x 20 cm, sheet, 25/pk |
| 10402096 |
BA79 Protran, 0.1 µm, blotting membrane, 30 cm x 3 m, roll, 1/pk |
|
|
| 10401316 |
BA83 Protran, 0.2 µm, blotting membrane, 82 mm, circle, 50/pk |
| 10402488 |
BA83 Protran, 0.2 µm, blotting membrane, 10.2 x 13.3 cm, sheet, fits Minifold I System, 10/pk |
| 10402493 |
BA83 Protran, 0.2 µm, blotting membrane, 6.3 x 22.8 cm, sheet, fits Minifold II System, 10/pk |
| 10402405 |
BA83 Protran, 0.2µm, blotting membrane, 15 x 15 cm, sheet, 5/pk |
| 10401465 |
BA83 Protran, 0.2 µm, blotting membrane, 15 x 20 cm, sheet, 10/pk |
| 10402452 |
BA83 Protran, 0.2 µm, blotting membrane, 20 x 20 cm, sheet, 5/pk |
| 10401391 |
BA83 Protran, 0.2 µm, blotting membrane, 20 x 20 cm, sheet, 25/pk |
| 10401380 |
BA83 Protran, 0.2 µm, blotting membrane, 30 x 60 cm, sheet, 5/pk |
| 10402480 |
BA83 Protran, 0.2 µm, blotting membrane, 33 x 56 cm, sheet, 5/pk |
| 10402468 |
BA83 Protran, 0.2 µm, blotting membrane, 15 cm x 3 m, roll, 1/pk |
| 10402495 |
BA83 Protran, 0.2 µm, blotting membrane, 20 cm x 3 m, roll, 1/pk |
| 10401396 |
BA83 Protran, 0.2 µm, blotting membrane, 30 cm x 3 m, roll, 1/pk |
|
|
| 10402506 |
BA85 Protran, 0.45 µm, blotting membrane, 25 mm, circle, 100/pk |
| 10402578 |
BA85 Protran, 0.45 µm, blotting membrane, 25 mm, circle, 1000/pk |
| 10401116 |
BA85 Protran, 0.45 µm, blotting membrane, 82 mm, circle, 50/pk |
| 10405316 |
BA85 Protran, 0.45 µm, blotting membrane, 82 mm, circle with 5 mm grid, 50/pk |
| 10402579 |
BA85 Protran, 0.45 µm, blotting membrane, 82.5 mm, circle, 50/pk |
| 10401164 |
BA85 Protran, 0.45 µm, blotting membrane, 87 mm, circle, 50/pk |
| 10401124 |
BA85 Protran, 0.45 µm, blotting membrane, 132 mm, circle, 25/pk |
| 10402525 |
BA85 Protran, 0.45 µm, blotting membrane, 132 mm, circle, 50/pk |
| 10401147 |
BA85 Protran, 0.45 µm, blotting membrane, 137 mm, circle, 25/pk |
| 10402548 |
BA85 Protran, 0.45 µm, blotting membrane, 137 mm, circle, 50/pk |
| 10402588 |
BA85 Protran, 0.45 µm, blotting membrane, 10.2 x 13.3 cm, sheet, fits Minifold I System, 10/pk |
| 10402593 |
BA85 Protran, 0.45 µm, blotting membrane, 6.3 x 22.8 cm, sheet, fits Minifold II System, 10/pk |
| 10401218 |
BA85 Protran, 0.45 µm, blotting membrane, 8.2 x 12 cm, sheet, 10/pk |
| 10402606 |
BA85 Protran, 0.45 µm, blotting membrane, 15 x 15 cm, sheet, 5/pk |
| 10401261 |
BA85 Protran, 0.45 µm, blotting membrane, 15 x 15 cm, sheet, 25/pk |
| 10402680 |
BA85 Protran, 0.45 µm, blotting membrane, 20 x 20 cm, sheet, 5/pk |
| 10401191 |
BA85 Protran, 0.45 µm, blotting membrane, 20 x 20 cm, sheet, 25/pk |
| 10402694 |
BA85 Protran, 0.45 µm, blotting membrane, 25 x 25 cm, sheet, 5/pk |
| 10401180 |
BA85 Protran, 0.45 µm, blotting membrane, 30 x 60 cm, sheet, 5/pk |
| 10402580 |
BA85 Protran, 0.45 µm, blotting membrane, 33 x 56 cm, sheet, 5/pk |
| 10402594 |
BA85 Protran, 0.45 µm, blotting membrane, 15 cm x 3 m, roll, 1/pk |
| 10401197 |
BA85 Protran, 0.45 µm, blotting membrane, 20 cm x 3 m, roll, 1/pk |
| 10401196 |
BA85 Protran, 0.45 µm, blotting membrane, 30 cm x 3 m, roll, 1/pk |
by williamsu | Aug 22, 2012 | Product News
3MM Chr
Whatman 3MM Chr paper is the world’s most widely used blotting paper. This acceptance and usage reflect the high quality, purity,and consistency that are relied upon by researchers doing Southern, Northern, and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) is used extensively in electrophoresis for lifting of sequencing gels.
GB003
A general purpose blotting paper (0.8 mm) made from pure raw materials with a high absorbency used as a membrane gel support. A thick paper recommended for the lysis/denaturation of colony or plaque lifts and Western blots.
GB005
A thick (1.5 mm) highly absorbent paper recommended for applications where fewer layers of blotting paper must still ensure a high capacity. Recommended for semi-dry blotting of proteins.
Features and benefits
- Whatman 3MM Chr Paper pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps.
- Manufactured and tested specifically for chromatographic and blotting techniques. This ensures the wicking capability and uniformity of capillary action that are important in obtaining clean and even transfers during blotting.
- Whatman 3MM Chr is considered the industry standard for blotting procedures.
- Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows ‘out-of-the-box’ usage and eliminates sheet-to-sheet variations.
| Ordering Information – Blotting Papers |
| Catalog Number |
Description |
|
|
| 3030-6185 |
3MM Chr sheets, 11 x 14 cm, 100/pk |
| 3030-6132 |
3MM Chr sheets, 12 x 14 cm, 100/pk |
| 3030-153 |
3MM Chr sheets, 15 x 17.5 cm, 100/pk |
| 3030-6188 |
3MM Chr sheets, 15 x 20 cm, 100/pk |
| 3030-221 |
3MM Chr sheets, 18 x 34 cm, 100/pk |
| 3030-861 |
3MM Chr sheets, 20 x 20 cm, 100/pk |
| 3030-6461 |
3MM Chr sheets, 26 x 41 cm, 100/pk |
| 3030-335 |
3MM Chr sheets, 31.5 x 35.5 cm, 100/pk |
| 3030-347 |
3MM Chr sheets, 35 x 43 cm, 100/pk |
| 3030-392 |
3MM Chr sheets, 35 x 45 cm, 100/pk |
| 3030-917 |
3MM Chr sheets, 46 x 57 cm, 100/pk |
| 3030-931 |
3MM Chr sheets, 58 x 68 cm, 100/pk |
| 3030-6189 |
3MM Chr sheets, 4 x 5.25 inches, 100/pk |
| 3030-6187 |
3MM Chr sheets, 6 x 8 inches, 100/pk |
| 3030-866 |
3MM Chr sheets, 8 x 10 inches, 100/pk |
| 3030-614 |
3MM Chr roll, 2 cm x 100 m, 1/pk |
| 3030-662 |
3MM Chr roll, 7.5 cm x 100 m, 1/pk |
| 3030-672 |
3MM Chr roll, 10 cm x 100 m, 1/pk |
| 3030-675 |
3MM Chr roll, 12.5 cm x 100 m, 1/pk |
| 3030-681 |
3MM Chr roll, 15 cm x 100 m, 1/pk |
| 3030-690 |
3MM Chr roll, 19 cm x 100 m, 1/pk |
| 3030-700 |
3MM Chr roll, 23 cm x 100 m, 1/pk |
| 3030-704 |
3MM Chr roll, 27 cm x 100 m, 1/pk |
|
|
| 10426880 |
GB003 blotting sheets, 10 x 10 cm, 50/pk |
| 10427810 |
GB003 blotting sheets, 15 x 15 cm, 100/pk |
| 10427812 |
GB003 blotting sheets, 15 x 20 cm, 100/pk |
| 10427813 |
GB003 blotting sheets, 16 x 18 cm, 100/pk |
| 10427818 |
GB003 blotting sheets, 20 x 20 cm, 100/pk |
| 10426890 |
GB003 blotting sheets, 30 x 60 cm, 25/pk |
| 10427826 |
GB003 blotting sheets, 46 x 57 cm, 100/pk |
| 10426892 |
GB003 blotting sheets, 58 x 60 cm, 50/pk |
|
|
| 10426972 |
GB005 blotting sheets, 15 x 15 cm, 25/pk |
| 10426981 |
GB005 blotting sheets, 20 x 20 cm, 25/pk |
| 10426994 |
GB005 blotting sheets, 58 x 58 cm, 25/pk |
Whatman 3MM Chr paper is the world’s most widely used blotting paper. This acceptance and usage reflect the high quality, purity,and consistency that are relied upon by researchers doing Southern, Northern, and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) is used extensively in electrophoresis for lifting of sequencing gels.
by williamsu | Aug 17, 2012 | Product News
When leading researchers and specialists find themselves amazed by specialty laboratory equipment, IKA® is typically involved. With samples and processes that will change our future, substances in all states of aggregation can be transformed into innovative products through experimentation and production. Mixed or crushed, tempered or distilled, in new compounds or reproducible results; from anti-aging cream to cement, a tissue sample or pioneering a new development, in the µ range or on an industrial scale, IKA® is the solution.
IKA® not only ensures the highest possible degree of precision and quality in results, but also demonstrates through its innovative design that a laboratory need not be boring and can make innovation visible.
State-of-the-art laboratory and analytical equipment is researched, designed, and tested at our central location in Staufen, Germany by more than 400 IKA® employees. In recent years, IKA® has committed itself to strengthen its leading position in the world market by developing innovative hotplate, magnetic, and overhead stirrers, as well as shakers, homogenizers, mills, rotary evaporators, calorimeters, and laboratory reactors. IKA® also helps enhance the laboratory and analytical application process by offering specially developed software.
New IKA hotplate made of ceramic glass which offers excellent chemical resistance.- Fixed safety circuit of 550 °C
– Hot Top indicator >> hot surface warning to prevent burns!
– Exact temperature setting via digital display (LED)
– Bushing according to DIN 12878 for connecting a contact thermometer, e.g. ETS-D5, enables precise temperature control
– Digital error code display
– Elevated control panel for protection against leaking liquids
| C-MAG HP 10 IKATHERM® |
| Number of heating points |
1 |
| Heat output |
1500 W |
| Heating temperature range |
50 – 500 °C |
| Heat control |
LED |
| Heat control accuracy |
10 ±K |
| Connection for ext. temperature sensor |
ETS-D 4 fuzzy |
| Control accuracy with sensor |
3 ±K |
| Temperature constancy in medium |
3 ±K |
| Heating rate (1 l H2O in H15) |
5 K/min |
| Set-up plate material |
ceramic |
| Set-up plate dimensions |
280 x 280 mm |
| Dimensions (W x H x D) |
300 x 105 x 415 mm |
| Weight |
6 kg |
| Permissible ambient temperature |
5 – 40 °C |
| Permissible relative moisture |
80 % |
| Protection class according to DIN EN 60529 |
IP 21 |
| Voltage |
230 V |
| Frequency |
50/60 Hz |
| Power input |
1505 W |
by williamsu | Aug 15, 2012 | Product News
KNF Neuberger LIQUIPORT® Diaphragm Liquid Pumps NF100 FT.18S
The LIQUIPORT diaphragm liquid pumps of the NF 100/1.100_.18 S series reliably pump neutral and aggressive liquids. A knob with LED makes it easy to vary the pumping
capacity. These pumps work quietly and with low vibrations.
Technical features:
- Acid, alkali, solvent resistant
- Self priming
- Dry running
- Maintenance-free
- Splashproof (IP 65)
- Flow rate manually adjustable
- Standby mode
Technical data: NF100FT.18 S
Flow rate (l/min)1) 0.2-1.3
Suction head (mWg) 3
Pressure head (mWg) 10
Connectors for tube (mm) ID 8
Permissible ambient temperature +5…+40 °C
Liquid temperature +5…+80 °C
Mains 100-230 V / 50-60 Hz
Motor protection IP 65
(splashproof)
Power P1 12 W
Weight 1.0 kg
Dimensions LxHxW (mm) 130/177/99
