Whatman Nytran™ nylon membranes are available in two formats. The Nytran N is moderately charged and the Nytran SuPerCharge (SPC) has a very high positive charge.

Nytran nylon
The Whatman Nytran nylon membrane is suitable for applications that require a lower charge. It is designed for Southern and Northern blotting as well as colony and plaque lifts and Dot-/Slot-Blots. Nytran N is compatible with isotopic and nonisotopic detection methods.

Nytran N membrane allows for excellent signal-to-noise ratios. The membrane is cast uniformly on both sides of a support matrix, demonstrating excellent symmetry. This gives the membrane the ability to lie flat without curling. Nytran N membrane is a highly consistent membrane with uniform pore size and distribution.It is available in 0.2 µm and 0.45 µm pore sizes for optimal retention of oligos and larger DNA fragments.

Nytran SuPerCharge (SPC)
Nytran SPC nylon membranes have a very high positive charge. Improvements in the manufacturing process result in a membrane with a higher density of nylon per unit area. The increased charge and greater nylon density provide increased binding sites for your samples.

Nytran SPC membranes show a very uniform pore size and pore distribution compared to typical nylon membranes. They are free of surface microvoids which are common in other membranes. These characteristics lead to greater reproducibility of results across a membrane and from blot to blot.

Nytran SPC membrane is cast uniformly on both sides of a support matrix, demonstrating excellent symmetry. This gives the membrane the ability to lie flat without curling.

With typical manufacturing techniques, increasing positive charge tends to increase background. Nytran SPC membranes are manufactured using a process that allows the combination of high positive charge with low background. Whether using radioactive or nonradioactive detection techniques, Nytran SPC consistently gives high signal with extremely low background.

Nytran binding capacity: > 400 µg/cm2; Nytran SuPerCharge: > 600 µg/cm2

Ordering Information – Nytran Nylon Membranes
Catalog Number Description
Nytran N Blotting Membranes
10416085 Nytran N, 0.2 µm nylon blotting membranes 20 x 20 cm sheets 10/pk
10416094 Nytran N, 0.2 µm nylon blotting membranes 20 cm x 3 m roll 1/pk
10416096 Nytran N, 0.2 µm nylon blotting membranes 30 cm x 3 m roll 1/pk
10416116 Nytran N, 0.45 µm nylon blotting membranes 82 mm circles 50/pk
10416194 Nytran N, 0.45 µm nylon blotting membranes 20 cm x 3 m roll 1/pk
10416196 Nytran N, 0.45 µm nylon blotting membranes 30 cm x 3 m roll 1/pk
Nytran SuPerCharge (SPC) Blotting Membranes
10416216 Nytran SPC, 0.45 µm nylon blotting membranes 82 mm circles 50/pk
10416224 Nytran SPC, 0.45 µm nylon blotting membranes 132 mm circles 50/pk
10416285 Nytran SPC, 0.45 µm nylon blotting membranes 20 x 20 cm sheets 10/pk
10416230 Nytran SPC, 0.45 µm nylon blotting membranes 11 x 14 cm sheets 10/pk
10416284 Nytran SPC, 0.45 µm nylon blotting membranes 15 x 15 cm sheets 10/pk
10416280 Nytran SPC, 0.45 µm nylon blotting membranes 30 x 60 cm sheets 5/pk
10416291 Nytran SPC, 0.45 µm nylon blotting membranes 22.2 x 22.2 cm sheets, macroarray membrane size 48/pk
10416294 Nytran SPC, 0.45 µm nylon blotting membranes 20 cm x 3 m roll 1/pk
10416296 Nytran SPC, 0.45 µm nylon botting membranes 30 cm x 3 m roll 1/pk